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ICLC catalog code: ATL02001
Continuous culture, grown as semiadherent
SPECIES: mouse, BALB/c (male)
DEPOSITOR: Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split subconfluent cultures 1:5-1:10 using trypsin/EDTA or scraper; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
The cells grow adherent, but some are loosely attached to the surface and others in suspension
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 91062702
- Cell 1978;15:261-267 - PMID: 79023065
- J Immunol 1977;119:950-954 - PMID: 77250888
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.