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SPECIES: rat, Wistar-Furth
DSMZ catalog code: ACC 108
Morphology: spindle-shaped, relatively small cells, growing adherently in monolayers
Description: rat brain tumor this clone is a bromodeoxyuridine-resistant mutant derived from cell line C6 (established from a nitrosourea-induced brain tumor in a Wistar-Furth strain rat in 1971) confirmed as rat with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. B. Hamprecht, Physiologisch-Chemisches Institut, Tuebingen, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS + 100 śM 5-bromodeoxyuridine split confluent cultures 1:4 to 1:10 every 4 days using trypsin/EDTA; let the cells not grow to complete confluence; seed out at ca. 4 x 106 cells/80 cm 2 at 37C with 5-10% CO2 about 2-3 x 107 cells/80 cm 2;
doubling time of about 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of NP, MDH)
- Int Rev Cytol 1977;49:99-170 - PMID: 16829
- Physiol Chem 1973;354:1633-1641
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.