Hep G2

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

ICLC catalog code: HTL95005
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 15 years old)
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichimotrypsin, haptoglobin, ceruloplasmin, plasminogen, C3,C4, C3 act., fibrinogen, alpha1-acid glycop., alpha2-HS glycoprotein, beta lipoprotein and retinol binding protein; xenobiotic metabolism, drug metabolism assays, toxicity screening, hepatocyte studies

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-3 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 17; FGA: 22,25; TH01: 9; D18S51: 13,14; D21S11: 29,31; D8S1179: 15,16) * isoenzymes (confirmed as human with MD, MPI)
Molecular authentication: Amelogenin: X,Y ; D18S51: 13,14 ; D21S11: 29,31 ; D8S1179: 15,16 ; FGA: 22,25 ; TH01: 9 ; VWA: 17

Availability in cell line catalogues: IZSBS: BS TCL79, ECACC: 85011430

Bibliographic references:

Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

See homonymous cell lines in other collections and catalogues.

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105