A4-840

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

A4-840 (mouse / mouse, hybrid, hybridoma)

SPECIES: mouse / mouse
TISSUE/ORGAN: hybrid
TUMOR: hybridoma

DSMZ catalog code: ACC 213
Morphology: single round cells, lightly adherent
Description: mouse hybridoma (anti-fiber/myosin) described as secreting a mouse monoclonal antibody (IgM) reacting with slow muscle fibers in rodent, chicken and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin confirmed as mouse with IEF of AST, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. S. M. Hughes, King's College, London, England
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids split saturated culture 1:5 every 3 days; seed out at about 0.3 x 106 cells/ml at 37C with 5% CO2 maximal density of about 1.5 x 106 cells/ml; doubling time of about 25 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * reverse transcriptase (reverse transcriptase negative)

Bibliographic references:

Virchows Arch B Cell Pathol 1993;63:335-343

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105