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SPECIES: rat, Wistar
ECACC catalog code: 88120601
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
Description: Rat Wistar sarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Plaque assay and virus studies: Rous sarcoma virus, MuLV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 42
Original tumour induced in outbred Wistar rats by intramuscular injection of Rous sarcoma virus. The cells have virus genome but do not normally release infectious virus. The XC plaque assay is an indirect method of detection for MuLV in cultured cells.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 165, IZSBS: BS TCL66, DSMZ: ACC 118, ICLC: ATL96013
- Folia Biol (Praha) 1962;8:221
- Nature 1960;186:980-981
- Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
- Virology 1970;42:1136-1139 - PMID: 4099080
See homonymous cell lines in other collections and catalogues.
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Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.