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ICLC catalog code: ATL96004
Continuous culture, grown as monolayer
SPECIES: mouse, BALB/c
Transformed by: tumor induced by subcutaneous injection of 3-methylcholanthrene Back To Chemical Agents' WebPage
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: tumorigenicity and cytotoxicity studies: highly sensitive to human cytotoxic monocytes, human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) after pretreatment with actinomycin D.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA, seed at 2-4 x 104 cells/cm2. 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Availability in cell line catalogues: ECACC: 94052601
- Blood 1985;65:8-14 - PMID: 3155490
- J Natl Cancer Inst 1984;72:23-29 - PMID: 6582301
- Proc Soc Exp Biol Med 1973;144:813-818 - PMID: 4543620
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.