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SPECIES: rat, Lewis
ECACC catalog code: 94011416
TRANSFORMED BY: RSV-33
Description: Rat Lewis fibrosarcoma
Viruses: See Properties
Depositor: Dr M Grofova/Dr S Blahova, Cancer Research Institute, Bratislava, SLOVAKIA (Originator)
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 40
Derived during co-culture of rat lewis embryonic fibroblasts with chicken sarcoma cells originally obtained from a tumour induced in vivo by RSV-33. The cells contain the viral genome but do not express virus specific RNA. The cells are tumourigenic in rats.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Folia Biol 1992;38:90 - PMID: 1326448
- Neoplasma 1991;38:617 - PMID: 1662779
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.