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SPECIES: mouse, NIH Swiss
ECACC catalog code: 89032007
Transfected by: Transfection Back To Transfection WebPage
Description: Mouse fibroblast with herpes TK gene
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe patent claims. US Patent No. 4,861,719
Properties: Applications: Transfection and virus studies: herpesvirus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Derived from TK- NIH/3T3 by co-transfection with packaging construct DNA (pPAM3) carried in pBR 322 and HSV TK gene in pBR 322. Mutations in pPAM3 including deletion of the retroviral packaging signal, all of the 3 LTR, part of the 5 LTR and the site for second-strand DNA synthesis, in the helper virus in this packaging cell line prevents helper virus production or transfer of packaging function. Introduction of retroviral vectors by transfection or infection results in the production of retrovirus virions with an amphotropic host range that have been used to transfer genes to humans and are capable of infecting cells of many mammalian species.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 9078, ICLC: HTL06006
- Mol Cell Biol 1986;6:2895-2902 - PMID: 3537716
- N Engl J Med 1990;232:570-578 - PMID: 2381442
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.