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SPECIES: mouse, BALB/c
DSMZ catalog code: ACC 43
Morphology: single, round, large cells in suspension
Description: mouse myeloma X63AG8.653 is a non-secreting clone derived from P3X63AG8 (a Balb/c myeloma) and is recommended as fusion parent for production of hybridomas confirmed as mouse with IEF of LDH, MDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM or RPMI 1640 + 10% FBS maintain at 0.1-1.0 x 106 cells/ml; optimal split ratio of 1:10 to 1:20 every 48 hours; seed out at about 1 x 106 cells/ml at 37C with 5% CO2 maximal density of ca. 1.5-2.0 x 106 cells/ml;
doubling time of about 30-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as mouse with IEF of LDH, MDH, NP) * reverse transcriptase (reverse transcriptase positive)
Availability in cell line catalogues: ATCC: CRL 1580
- J Immunol 1979;123:1548-1550 - PMID: 113458
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.