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For information, get in touch with: Dott.ssa Paola Visconti
  P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

OMEGA-E (mouse, NIH Swiss, embryo)

SPECIES: mouse, NIH Swiss

DSMZ catalog code: ACC 253
Morphology: cells grow as adherent fibroblastic monolayers
Description: NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by 'codon wobbling'; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as mouse with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. W. Dirks, DSMZ, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

Bibliographic references:

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105