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SPECIES: rat, Wistar
ECACC catalog code: 93012901
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Rat Wistar bladder tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Maturation of epidermoid carcinoma
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 2.5x1,000,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from a male Wistar rat bladder tumour induced with N-butyl-N-(4-hydroxybutyl) nitrosamine. The histotypic differentiation normally displayed by these cells is inhibited by vitamin A and gives rise to the appearance of giant cells.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1655
- Cancer Res 1975;35:1873-1879 - PMID: 1093679
- Cancer Res 1978;38:1223 - PMID: 639055
- Cell Motil 1982;4:333 - PMID: 7151140
- J Natl Cancer Inst 1971;47:979-985
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.