NAMALWA.CSN/70

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

NAMALWA.CSN/70 (human, African, lymphoma, Burkitt)

SPECIES: human, African
TUMOR: lymphoma, Burkitt

DSMZ catalog code: ACC 70
Morphology: lymphoblastoid, single cells or small clusters, in suspension
Description: human Burkitt's lymphoma established from the tumor mass of an African child with Burkitt's lymphoma; this variant subline from the original NAMALWA (DSM ACC 24) was described to be characterized by differential expression of immunoglobulin and c-myc genes and of certain cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase positive; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Guy, Western General Hospital, Edinburgh, UK
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml at 37C with 5% CO2 maximal density at about 2.0 x 106 cells/ml; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (pseudotriploid karyotype with 2% polyploidy; 69(65-72)<3n>; NAMALWA marker chromosomes present) * DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as NAMALWA) * immunological analysis (CD3- CD10+ CD13(+) CD19+ CD37+ DR+ cyIgM/IgG-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase positive (RDDP assay))

Bibliographic references:

Int J Cancer 1990;45:109-118 - PMID: 2298494

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105