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SPECIES: mouse, BALB/c
ECACC catalog code: 95090612
TRANSFORMED BY: SV40
Description: Mouse alveolar macrophage, SV40 transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Phagocytosis studies, plaque assays, IL-1 assays
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The MH-S cell line was obtained from a 7 week-old male BALB/C J mouse by bronchoalveolar lavage followed by SV-40 transformation of an adherent cell enriched population of alveolar macrophages. Many properties of alveolar macrophages are expressed with the cells being phagocytic, esterase positive and peroxidase negative. The intracellular T-antigen and cell surface Ia and Mac-1 antigens are expressed as well as Fc receptors. IL-1 is constitutively expressed and can be increased by stimulation with lipopolysaccharides (LPS). A cellulose dependant suppression of the in vitro plaque-forming cell (PFC) i-espouse by MH-S cells has also been reported.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2019, IZSBS: BS CL 141
- J Leukoc Biol 1989;46:119
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
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Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.