KB

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

KB (human, Caucasian, cervix, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: cervix
TUMOR: carcinoma

DSMZ catalog code: ACC 136
Morphology: adherent cells, epithelial-like, growing as monolayers
Susceptibility to viruses: adenovirus 3 * herpes simplex * influenza * poliovirus 1 * vaccinia
Description: human cervix carcinoma (derivative of HELA) originally described as 'epidermoid carcinoma established from the mouth of a Caucasian man in 1954'; however, as shown by DSMZ DNA fingerprinting (see below) this cell line must be considered an HELA subclone (human cervix carcinoma, see HELA DSM ACC 57) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + MEM non-essential amino acids + 0.075% Na-bicarbonate split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA at 37C with 5% CO2 about 5-10 x 106 cells/80 cm 2 flask; frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypertriploid karyotype with 8% polyploidy; 76(73-78)<3n>) * DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as HELA) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin++ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 17, IZSBS: BS TCL34, ECACC: 94050408

Bibliographic references:

Arch Virol 1980;66:309 - PMID: 7447707
In Vitro 1978;14:469-475 - PMID: 566722
Nature 1976;259:211-213 - PMID: 1250349
Proc Soc Exp Biol Med 1955;89:362
Proc Soc Exp Biol Med 1965;120:361 - PMID: 4285453