COLO-818

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

COLO-818 (human, melanoma)

SPECIES: human
TUMOR: melanoma

DSMZ catalog code: ACC 81
Morphology: adherent growth in monolayers, mostly spindle-like, some cells with cellular processes, 1-5% very big cells, no strict contact inhibition
Description: human melanoma established from malignant melanoma confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture about 1:6 once a week using trypsin/EDTA; seed out at about 1 x 106 cells/ml at 37C with 5% CO2 cell harvest of about 8 x 106 cells/175 cm 2; doubling time of about 60 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)

Availability in cell line catalogues: ATCC: HTB 45

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
Oncogene 1994;9(3):939-942 - PMID: 8108138
Proc Natl Acad Sci U S A 1987;84(22):7972-7976 - PMID: 2825172

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105