HEP-G2

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

HEP-G2 (human, liver, carcinoma, hepatocellular)

SPECIES: human
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular

DSMZ catalog code: ACC 180
Morphology: adherent, epithelial-like cells growing as monolayers and in small aggregates
Description: human hepatocellular carcinoma established from the tumor tissue of a 15-year-old Argentine boy with hepatocellular carcinoma in 1975; cell line is patented; cells were described to not harbor a hepatitis B virus genome; cells reportedly produce a variety of proteins: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement (C3, C4), C3 activator, fibrinogen, alpha1-acid glycoprotein, alpha2-HS glycoprotein, ss-lipoprotein, retinol binding protein confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from ATCC (HB 8065), Rockville, Maryland, USA
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/25 cm 2 ; after thawing, initially to be cultured with 20% FBS at 37C with 5% CO2 cell harvest of about 25-30 x 106 cells/80 cm 2 ,; doubling time of ca. 50-60 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype; 52(47-54)<2n>XY, +2, +14, +17, +20, +2m, t(1;21)(p22.2;p11/12), i(17q)/ der(17)t(17;17)(p11;q11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin ++ (100%) desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HB 8065

Bibliographic references:

In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
J Lipid Res 1986;27:858 - PMID: 3021884
Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105