H25B10

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

H25B10 (mouse / mouse, hybridoma)

SPECIES: mouse / mouse
TUMOR: hybridoma

DSMZ catalog code: ACC 98
Morphology: single round to oval cells in suspension
Description: mouse hybridoma (anti hepatitis B) established by fusion of P3-NSI/1-AG4-1 mouse myeloma cells with spleen cells from a mouse immunized with hepatitis B surface antigen; a monoclonal antibody (IgG 1 ) against hepatitis B surface antigen is produced; patented cell line (= ATCC CRL 8017) confirmed as mouse with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS cells are difficult to culture! maintain at 0.5-1.0 x 105 cells/ml; split 1:4 every 2-3 days at 37C with 5% CO2 maximal density at about 1-2 x 106 cells/ml; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: reverse transcriptase (reverse transcriptase negative)

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105