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SPECIES: human, Caucasian
ECACC catalog code: 95032301
Description: Human Caucasian melanoma
Viruses: See Properties
Depositor: Dr B Souberbielle, Prof A G Dalgleish, Division of Oncology, C.M.S. St. George's Hospital Medical School, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Carcinogenesis studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10%FBS (Heat Inactivated). Split confluent cultures 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 60-64
GR-M was obtained from the thorax of a 36 year old male with skin metastases having received no treatment at the time of the biopsy. The cells express HLA class II and the melanoma associated antigen P97. The expression of epithelial cell kinase (ECK), a receptor protein tyrosine kinase, was also detected.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cancer Res 1995;55:2528 - PMID: 7780963
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.