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DSMZ catalog code: ACC 116
Morphology: epithelial cells growing as monolayers
Description: human cervix carcinoma (derivative of HELA) these cells were cloned from the GIRARDI HEART cell line (ATCC CCL 27, passage 623) in 1981 and were thought to 'represent human heart cells'; however, isoenzyme analysis (Nelson-Rees et al., Science 191: 96-97, 1976) and DNA fingerprinting at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of HELA; cells were described to be sensitive to various toxins and hormones confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. K. E. von Milczweski, Bundesanstalt fuer Milchforschung, Institut fuer Mikrobiologie, Kiel, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
45% Medium 199 (with Hank's salts) and 45% RPMI 1640 + 10% FBS split confluent culture 1:3 to 1:6 every 3-4 days using trypsin/EDTA at 37C with 5% CO2 about 10 x 106 cells/80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as HELA) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- Proc V International Symposium on Mycotoxins and Phycotoxins 1982
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.