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ICLC catalog code: HTL97013
Continuous culture, grown as monolayer
SPECIES: human (male, 53 years old) Karyology: hypertriploid; n = 80, over 20 marker chromosomes per cell
non tumorigenic in anti thymocyte
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Seed at 2-4x104 cells/cm2; split confluent cultures 1:4-1:8 using trypsin/EDTA. 37C, 5% CO2. Passage: 318, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, NP)
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.