SPECIES: human, African
TUMOR: lymphoma, Burkitt
DSMZ catalog code: ACC 78
Morphology: single round cells in suspension
Endogenous viruses: contains Epstein-Barr
Description: human Burkitt's lymphoma established from the Burkitt's lymphoma (left orbital biopsy) of a 16-year-old African boy in 1967; cells were described to be EBV-positive and to express mRNA for proto-oncogene bcl-2; this cell line has been assigned by the ZKBS (German Central Commission for Biological Safety) to risk category 2 confirmed as human with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; split ratio 1:2 to 1:4 every 2-4 days; cells may grow slowly initially; cell culture may be started in 24-well-plates and later transfered to flasks; seed out at ca. 0.5-1 x 106 cells/ml at 37C with 5% CO2 maximal density at about 1.7 x 106 cells/ml;
doubling time of about 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid with 20% polyploidy; 46(45-48)<2n>XY/XXY, +7, -9, t(8;14)(q24;q32)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ smIgG- smIgM+ sm+ sm-) * isoenzymes (confirmed as human with IEF of AST, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, ICLC: HTL95024, IZSBS: BS TCL 104
Bibliographic references:
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
- Clin Exp Immunol 1976;25:367-368 - PMID: 963908
- Int J Cancer 1977;19:334-337
- Tissue Antigens 1978;11:96-112 - PMID: 77067
SPECIES: human, Black
TISSUE/ORGAN: blood
TUMOR: lymphoma, Burkitt
ECACC catalog code: 85011437
Morphology: Lymphoblast
Endogenous viruses: contains Epstein-Barr
Description: Human Negroid Burkitt's lymphoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and Virus studies: EBV used to study leukemogenesis mechanisms.
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37C. Will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals.
Karyotype: 2n = 46, diploid
Derived from 16-yr old male Negro. Positive for EBNA, carries EBV markers, Complement Receptors, surface bound immunoglobulin and surface markers for Fc fragment of IgG. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 213, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104
Bibliographic references:
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
- Clin Exp Immunol 1976;25:367-368 - PMID: 963908
- Int J Cancer 1977;19:334-337
- Tissue Antigens 1978;11:96-112 - PMID: 77067
ICLC catalog code: HTL95024
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old)
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
Endogenous viruses: contains Epstein-Barr
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: complement receptor; Fc receptor; tumor markers; target line; differentiation; antitumour testing; tumorigenicity; virology
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Maintain cultures between 2 and 9 x 105 cells/ml; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with MD, NP)
Availability in cell line catalogues: ECACC: 85011437, DSMZ: ACC 78, IZSBS: BS TCL 104
Bibliographic references:
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
- Clin Exp Immunol 1976;25:367-368 - PMID: 963908
- Int J Cancer 1977;19:334-337
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- Tissue Antigens 1978;11:96-112 - PMID: 77067
IZSBS catalog code: BS TCL 104
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old) Karyology: 2n = 46 in 50 cells
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
DEPOSITOR: obtained from ATCC
PROPERTIES: well characterized as B lymphoblast cell line employed in studies on leukemogenesis mechanisms
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL-213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024
Bibliographic references:
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old)
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
Endogenous viruses: contains Epstein-Barr
PROPERTIES: complement receptor; Fc receptor; tumor markers; target line; differentiation; antitumour testing; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104
Bibliographic references:
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
- Clin Exp Immunol 1976;25:367-368 - PMID: 963908
- Int J Cancer 1977;19:334-337
- Tissue Antigens 1978;11:96-112 - PMID: 77067
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old)
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
Endogenous viruses: contains Epstein-Barr
PROPERTIES: complement receptor; Fc receptor; tumor markers; target line; differentiation; antitumour testing; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104
Bibliographic references:
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
- Clin Exp Immunol 1976;25:367-368 - PMID: 963908
- Int J Cancer 1977;19:334-337
- Tissue Antigens 1978;11:96-112 - PMID: 77067
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old)
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
Endogenous viruses: contains Epstein-Barr
PROPERTIES: complement receptor; Fc receptor; tumor markers; target line; differentiation; antitumour testing; tumorigenicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104
Bibliographic references:
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
- Clin Exp Immunol 1976;25:367-368 - PMID: 963908
- Int J Cancer 1977;19:334-337
- Tissue Antigens 1978;11:96-112 - PMID: 77067
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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