DAUDI's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

DAUDI (human, Black, lymphoma, Burkitt)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old)
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
Endogenous viruses: contains Epstein-Barr
PROPERTIES: complement receptor; Fc receptor; tumor markers; target line; differentiation; antitumour testing; tumorigenicity; virology

Available in the following LABORATORY:

Istituto Nazionale Riposo e Cura Anziani (INRCA) (ANIIR, Ancona)
mycoplasma negative
Validated by: immunofluorescence * isoenzymes (G6PD type B)

Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104

Bibliographic references:

Cancer Res 1968;28:1300-1310 - PMID: 4174339
Clin Exp Immunol 1976;25:367-368 - PMID: 963908
Int J Cancer 1977;19:334-337
Tissue Antigens 1978;11:96-112 - PMID: 77067

DAUDI (human, African, lymphoma, Burkitt)

SPECIES: human, African
TUMOR: lymphoma, Burkitt

DSMZ catalog code: ACC 78
Morphology: single round cells in suspension
Endogenous viruses: contains Epstein-Barr
Description: human Burkitt's lymphoma established from the Burkitt's lymphoma (left orbital biopsy) of a 16-year-old African boy in 1967; cells were described to be EBV-positive and to express mRNA for proto-oncogene bcl-2; this cell line has been assigned by the ZKBS (German Central Commission for Biological Safety) to risk category 2 confirmed as human with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; split ratio 1:2 to 1:4 every 2-4 days; cells may grow slowly initially; cell culture may be started in 24-well-plates and later transfered to flasks; seed out at ca. 0.5-1 x 106 cells/ml at 37C with 5% CO2 maximal density at about 1.7 x 106 cells/ml; doubling time of about 30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid with 20% polyploidy; 46(45-48)<2n>XY/XXY, +7, -9, t(8;14)(q24;q32)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ smIgG- smIgM+ sm+ sm-) * isoenzymes (confirmed as human with IEF of AST, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, ICLC: HTL95024, IZSBS: BS TCL 104

Bibliographic references:

Cancer Res 1968;28:1300-1310 - PMID: 4174339
Clin Exp Immunol 1976;25:367-368 - PMID: 963908
Int J Cancer 1977;19:334-337
Tissue Antigens 1978;11:96-112 - PMID: 77067

DAUDI (human, Black, lymphoma, Burkitt)

ICLC catalog code: HTL95024
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old)
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
Endogenous viruses: contains Epstein-Barr
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: complement receptor; Fc receptor; tumor markers; target line; differentiation; antitumour testing; tumorigenicity; virology

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Maintain cultures between 2 and 9 x 105 cells/ml; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with MD, NP)

Availability in cell line catalogues: ECACC: 85011437, DSMZ: ACC 78, IZSBS: BS TCL 104

Bibliographic references:

Cancer Res 1968;28:1300-1310 - PMID: 4174339
Clin Exp Immunol 1976;25:367-368 - PMID: 963908
Int J Cancer 1977;19:334-337
J Exp Med 1976;143:1528-1533 - PMID: 1083890
Tissue Antigens 1978;11:96-112 - PMID: 77067

DAUDI (human, Black, lymphoma, Burkitt)

IZSBS catalog code: BS TCL 104
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human, Black (male, 16 years old) Karyology: 2n = 46 in 50 cells
TUMOR: lymphoma, Burkitt
tumorigenic in nude mice
DEPOSITOR: obtained from ATCC
PROPERTIES: well characterized as B lymphoblast cell line employed in studies on leukemogenesis mechanisms

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MPI, PEPB, AST)

Availability in cell line catalogues: ATCC: CCL-213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024

Bibliographic references:

Cancer Res 1968;28:1300-1310 - PMID: 4174339

DAUDI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104

Bibliographic references:

Cancer Res 1968;28:1300-1310 - PMID: 4174339
Clin Exp Immunol 1976;25:367-368 - PMID: 963908
Int J Cancer 1977;19:334-337
Tissue Antigens 1978;11:96-112 - PMID: 77067

DAUDI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

Centro Trapianto Midollo Osseo e Divisione Ematologia (PSHTM, Pesaro)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + Antibiotics + 1mM Na pyruvate; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104

Bibliographic references:

Cancer Res 1968;28:1300-1310 - PMID: 4174339
Clin Exp Immunol 1976;25:367-368 - PMID: 963908
Int J Cancer 1977;19:334-337
Tissue Antigens 1978;11:96-112 - PMID: 77067

DAUDI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma positive

Availability in cell line catalogues: ATCC: CCL 213, ECACC: 85011437, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104

Bibliographic references:

Cancer Res 1968;28:1300-1310 - PMID: 4174339
Clin Exp Immunol 1976;25:367-368 - PMID: 963908
Int J Cancer 1977;19:334-337
Tissue Antigens 1978;11:96-112 - PMID: 77067

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105