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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

CPAE (bovine, endothelium, pulmonary artery)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 23; Karyotype: 2n = 60, diploid
  Established from the main stem pulmonary artery of a young, female cow (Bos taurus). The cells have angiotensin-converting enzyme activity. BVDV positive.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 209, IZSBS: BS CL21

Bibliographic references:

• J Clin Microbiol 1988;26:2324 - PMID: 2853175
  

CPAE (bovine, endothelium, pulmonary artery)

Available in the following LABORATORY:

• Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
  MEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3
  

Availability in cell line catalogues: ATCC: CCL 209, IZSBS: BS CL21, ECACC: 86111401

Bibliographic references:

• J Clin Microbiol 1988;26:2324 - PMID: 2853175