SPECIES: monkey, African green
TISSUE/ORGAN: kidney
DSMZ catalog code: ACC 60
Morphology: fibroblast-like cells growing as monolayers
TRANSFORMED BY: SV40
Description: African green monkey kidney derived from CV-1, a simian cell line (cercopithecus aethiops), by transformation with an origin-defective mutant of SV-40; cells were described to support the growth of SV-40 viruses; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as of primate origin with IEF of LDH, MPI, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institute, Insel Riems, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm 2 ; cells are growing slowly at 37C with 5% CO2 about 2-3 x 106 cells/80 cm 2;
doubling time of ca. 35-48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (simian hypodiploid karyotype with 25% polyploidy; 57(53-58)<2n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile (identical to COS-1)) * isoenzymes (confirmed as of primate origin with IEF of LDH,MPI,NP,PEP B)
Availability in cell line catalogues: ATCC: CRL 1651, IZSBS: BS CL103, ECACC: 87021302, ICLC: ATL96005
Bibliographic references:
- Cell 1981;23:175-182 - PMID: 6260373
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
ECACC catalog code: 87021302
Morphology: Fibroblast
TRANSFORMED BY: SV40
Description: Monkey African green kidney, SV40 transformed
Viruses: See Properties
Depositor: Dr H R B Pelham, MRC Laboratory of Molecular Biology, Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus replication studies: SV40
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 5;
Derived from CV-1simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1651, IZSBS: BS CL103, DSMZ: ACC 60, ICLC: ATL96005
Bibliographic references:
- Cell 1981;23:175-182 - PMID: 6260373
ICLC catalog code: ATL96005
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Original line: CV-1
TRANSFORMED BY: 25
DEPOSITOR: Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen.
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as monkey with MD, NP)
Availability in cell line catalogues: IZSBS: BS CL103, ECACC: 87021302, DSMZ: ACC 60
Bibliographic references:
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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