SPECIES: monkey, African green
TISSUE/ORGAN: kidney
DSMZ catalog code: ACC 63
Morphology: fibroblast-like, adherent monolayers
TRANSFORMED BY: SV40
Description: African green monkey kidney established from CV-1 Simian cells (cercopithecus aethiops) which were transformed by an origin-defective mutant of SV-40; cells are host cells for the propagation of pure populations of recombinant SV virus; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as of non-human primate origin with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HCV-, HBV-, HIV-, HTLV-I/II-
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institute, Insel Riems, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent cultures 1:3 to 1:10 every 3-7 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 about 5 x 106 cells/80 cm 2;
doubling time of ca. 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid simian karyotype with 4% polyploidy; 102(95-108)<4n>; structural rearrangements present) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile (identical to COS-7)) * isoenzymes (confirmed as of primate origin with IEF of NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1650, IZSBS: BS CL95, ECACC: 88031701, ICLC: ATL01002
Bibliographic references:
- Cell 1981;23:175-182 - PMID: 6260373
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
ECACC catalog code: 88031701
Morphology: Fibroblast
TRANSFORMED BY: SV40
Description: Monkey African green kidney, SV40 transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus replication studies: SV40
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Do not let cells become confluent in order to prevent syncytia formation.
Passages: 20;
Derived from CV-1 Simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1650, IZSBS: BS CL95, DSMZ: ACC 63, ICLC: ATL01002
Bibliographic references:
- Cell 1981;23:175-182 - PMID: 6260373
ICLC catalog code: ATL01002
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Original line: CV-1
TRANSFORMED BY: 25
DEPOSITOR: Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: This line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:10 using trypsin/EDTA; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: PCR analysis (confirmed as African green monkey with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: IZSBS: BS CL95, ECACC: 88031701, DSMZ: ACC 63
Bibliographic references:
IZSBS catalog code: BS CL95
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Original line: CV-1
TRANSFORMED BY: 25
PROPERTIES: transfection; virus replication
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1650, ECACC: 88031701, DSMZ: ACC 63, ICLC: ATL01002
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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