CHO-K1's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

CHO-K1 (hamster, Chinese, ovary)

SPECIES: hamster, Chinese
TISSUE/ORGAN: ovary

DSMZ catalog code: ACC 110
Morphology: adherent, fibroblastoid cells, may be induced to undergo additional differentiation
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)
Description: Chinese hamster ovary cells subclone from parental CHO cell line that was initiated from an ovary biopsy of an adult Chinese hamster in 1957 confirmed as hamster with IEF of NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. P. E. Bryant, University of St. Andrews, St. Andrews, Fife, UK
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Ham's F12 + 10% FBS split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out about 10 5 - 10 6 cells in an 80 cm 2 flask; cells grow up in 3-4 days to confluence at 37C with 5% CO2 about 10 x 106 cells/80 cm 2 or 3 x 10 6 cells/25 cm 2; doubling time of ca. 24 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid with 12% polyploidy; 22(20-23); exhibits distinctive CHO karyology) * DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as CHO-DHFR) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

SPECIES: hamster, Chinese
TISSUE/ORGAN: ovary

ECACC catalog code: 85051005
Morphology: Epithelial
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)
Description: Hamster Chinese ovary
Viruses: See Properties
Depositor: Dr D Newell, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Nutritional and gene expression studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 17; Karyotype: Hypodiploid, modal No 20
A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 61, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

ICLC catalog code: ATL98003
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: hamster, Chinese (female, adult)
TISSUE/ORGAN: ovary
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: The cells have an absolute requirement for L-proline

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as hamster with MD, NP)

Availability in cell line catalogues: ECACC: 85051005, DSMZ: ACC 110

Bibliographic references:

J Exp Med 1958;108:945

CHO-K1 (hamster, Chinese, ovary)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: hamster, Chinese Karyology: hypodiploid, modal number 20
TISSUE/ORGAN: ovary
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)
PROPERTIES: genetics; nutrition; transfection; toxicity screening; cell biology; virology; cytotoxicity; bacterial cytotoxicity; gene expression

Available in the following LABORATORY:

Istituto di Scienze Farmacologiche, Universita' degli Studi (MIUFC, Milano)
Ham's F12 + DMEM (1:1) + 10% FBS + Antibiotics; Freezing medium: Culture medium + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 1-4 , mycoplasma negative
Validated by: biochemical assays * cellular assays

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Available in the following LABORATORY:

Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2
Proline-requiring

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Available in the following LABORATORY:

Dip. Biologia Molecolare, Universita' degli Studi (SIUVI, Siena)
MEM modified + 10% FBS + Antibiotics + NaHCO3; Freezing medium: MEM modified + 20% FBS + 7.5% DMSO; 37C, 5% CO2, split 1-4 , mycoplasma negative, enzyme immunoassay (EIA)
Validated by: chromosome analysis

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105