CHO's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

CHO (hamster, Chinese, ovary)

Continuous culture, grown as monolayer
fibroblast-like and epithelial-like morphology
SPECIES: hamster, Chinese (female)
TISSUE/ORGAN: ovary
PROPERTIES: genetics; toxicity screening; nutrition; gene expression

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro (GEIMG, Genova)
Ham's F10 + 10% FBS; Freezing medium: Ham's F10 + 15% FBS + 6% DMSO; 37C, 5% CO2
Available upon request to original source: Dott.ssa M. Bignami, Istituto Superiore Sanita', Roma, Italy
Validated by: chromosome analysis * plating efficiency

Availability in cell line catalogues: ECACC: 85050302, ICLC: ATL95003

Bibliographic references:

J Exp Med 1958;108:945

CHO (hamster, Chinese, ovary)

ICLC catalog code: ATL95003
Continuous culture, grown as monolayer
fibroblast-like and epithelial-like morphology
SPECIES: hamster, Chinese (female)
TISSUE/ORGAN: ovary
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: genetics; toxicity screening; nutrition; gene expression

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA;seed at 1-3x104 cells/cm2;37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as hamster with AST, MD)

Availability in cell line catalogues: ECACC: 85050302

Bibliographic references:

J Exp Med 1958;108:945

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105