A-498's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

A-498 (human, kidney, carcinoma)

SPECIES: human
TISSUE/ORGAN: kidney
TUMOR: carcinoma

DSMZ catalog code: ACC 55
Morphology: epithelial-like, adherent cells growing as monolayers
Description: human kidney carcinoma established from the kidney carcinoma of a 52-year-old man in 1973 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS split confluent cultures 1:3 to 1:5 every 3-5 days using trypsin/EDTA; seed out at ca. 1.0 x 106 cells/80 cm 2 at 37C with 5% CO2 about 2-3 x 106 cells/80 cm 2; doubling time of > 60 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypertriploid with 7% polyploidy; 72(68-72)<3n>XX, -X, +2, -3, -6, +7, +7, +9, -11, -14, +17, -18, -20, +21, +3m, dm/r (31%); der(1)t(1;1)(p32;q12), der(2)t(2;3)(q37;q13), i(8q); sideline with der(3)t(3;3)(q27;p14)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofil- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative)

Availability in cell line catalogues: ATCC: HTB 44, IZSBS: BS TCL 111, ICLC: HTL04005

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A-498 (human, kidney, carcinoma)

IZSBS catalog code: BS TCL 111
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (female, 52 years old) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: kidney
TUMOR: carcinoma
tumorigenic in nude mice
DEPOSITOR: obtained from ATCC
PROPERTIES: expressing xenobiotic metabolizing enzymes

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 34 , mycoplasma negative, culture
Forming undifferentiated carcinomas in nude mice.
Validated by: isoenzymes (confirmed as human with NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: HTB-44, ATCC: HTB-44, DSMZ: ACC 55, ICLC: HTL04005

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A-498 (human, kidney, carcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 52 years old)
TISSUE/ORGAN: kidney
TUMOR: carcinoma
tumorigenic in nude mice

Available in the following LABORATORY:

Dip. Biochimica Biotecnologie Mediche, Universita' degli Studi (NAUBC, Napoli)
MEM + 10% FBS + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: HTB 44, DSMZ: ACC 55, IZSBS: BS TCL 111, ICLC: HTL04005

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105