SPECIES: human
TISSUE/ORGAN: kidney, embryonic
ECACC catalog code: 85120602
Morphology: Epithelial
TRANSFORMED BY: adenovirus 5
Susceptibility to viruses: adenovirus * astrovirus
Description: Human Embryo Kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Horse Serum (HS) or 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Cells may take up to 7 days to attach after resuscitation and sub culture.
Passages: 36;
Karyotype: 2n = 46, hypotriploid, Modal No. 64
Transformed with sheared human Ad5 DNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Ad5 and for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2%. The der (1)t(1;15) (q42;q13), der(19)t(3;19),der(12)t(8;12) (q22;p13) and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+ and a single Xp+ in most cells. Detach at room temp; may take up to seven days to reattach.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1573, ICLC: HTL03003, DSMZ: ACC 305
Bibliographic references:
- J Gen Virol 1977;36:59 - PMID: 886304
- Virology 1977;77:319 - PMID: 841862
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human Karyology: contain and express the transforming genes of Ad5
TISSUE/ORGAN: kidney, embryonic
TRANSFORMED BY: 6
Susceptibility to viruses: adenovirus * astrovirus
PROPERTIES: virology; transformation; virus titration
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1573, ECACC: 85120602, ICLC: HTL03003, DSMZ: ACC 305
Bibliographic references:
- J Gen Virol 1977;36:59 - PMID: 886304
- Virology 1977;77:319 - PMID: 841862
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human Karyology: contain and express the transforming genes of Ad5
TISSUE/ORGAN: kidney, embryonic
TRANSFORMED BY: 6
Susceptibility to viruses: adenovirus * astrovirus
PROPERTIES: virology; transformation; virus titration
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1573, ECACC: 85120602, ICLC: HTL03003, DSMZ: ACC 305
Bibliographic references:
- J Gen Virol 1977;36:59 - PMID: 886304
- Virology 1977;77:319 - PMID: 841862
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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