5637's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

5637 (human, bladder, carcinoma)

SPECIES: human
TISSUE/ORGAN: bladder
TUMOR: carcinoma

DSMZ catalog code: ACC 35
Morphology: epithelial-like adherent cells
Description: human urinary bladder carcinoma established from the primary bladder carcinoma of a 68-year-old man in 1974; cells were described to produce several growth factors (e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. K. Welte, Medical School of Hannover, Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures 1:4 to 1:5 using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 8-10 x 10 6 cells/80 cm 2; doubling time of ca. 24 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotriploid with 17% polyploidy; 62(47-64)<3n>XX, -Y, -4, -5, -10, -13,-13, -15, -18, -19, -21, -21, +4mar, der(1)t(1;?)(p21;?), der(3)t(3;?)(p22;?), del(6)(q23), der(9)t(9;?)(p12;?), der (11)t(5;11)(q14;p12), ?der(12)t(12;) * cytogenetics 2 (i(13q), i(14q), der(17)t(17;?)(p11/12;?), del(22)(q13); carries large submetacentric marker with q arm resembling tandem dup(7); 9p12 is a recurrent breakpoint in bladder carcinoma.) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HTB 9, ECACC: 90110524, ICLC: HTL01017

Bibliographic references:

Proc Natl Acad Sci U S A 1985;82:1526-1530 - PMID: 3871951

5637 (guinea pig, 2/N, fetal)

SPECIES: guinea pig, 2/N
TISSUE/ORGAN: fetal

ECACC catalog code: 90110524
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Guinea pig fetal cells transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5 x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 32; Karyotype: Diploid
Derived from a female foetal carcass of a 2/N guinea pig. The cells were treated with benzopyrene for 7 days and a clone selected at the 20th passage. The cells are tumourigenic in guinea pigs.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1405, DSMZ: ACC 35, ICLC: HTL01017

Bibliographic references:

Cancer Res 1975;35:1035 - PMID: 1116142
Cancer Res 1975;35:1045 - PMID: 1116143
Cancer Res 1976;36:128 - PMID: 1247991

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105