SPECIES: dog
TISSUE/ORGAN: thymus
ECACC catalog code: 90110521
Morphology: Epithelial
Susceptibility to viruses: calicivirus * feline rhinotracheitis * herpes simplex * parvovirus * pseudorabies * reovirus * retroviruses
Description: Canine thymus
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: C type viruses, xenotrophic viruses
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 0.02% EDTA: 5% CO2; 37C.
Passages: 62;
Used widely for the growth of C type viruses obtained from a variety of species including cat, baboon and humans. Also permissive for xenotrophic viruses found in BALB/c and AKR mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1430, IZSBS: BS CL13, ICLC: AL97003
Bibliographic references:
- Am J Vet Res 1983;44:865 - PMID: 6869994
- Arch Virol 1983;76:257 - PMID: 6307228
- Avian Dis 1984;28:216 - PMID: 6721794
- In Vitro 1976;12:665 - PMID: 190163
- In Vitro 1977;9:176
ICLC catalog code: AL97003
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: dog
TISSUE/ORGAN: thymus
Susceptibility to viruses: calicivirus * feline rhinotracheitis * herpes simplex * parvovirus * pseudorabies * reovirus * retroviruses
DEPOSITOR: Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Permissive for xenotropic viruses found in Balb/c and AKR mice; C-type viruses growth
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 Passage: 9 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as dog with G6PD, MD.)
Availability in cell line catalogues: IZSBS: BS CL13, ECACC: 90110521
Bibliographic references:
- Am J Vet Res 1983;44:865 - PMID: 6869994
- In Vitro 1976;12:665 - PMID: 190163
- In Vitro 1977;9:176
IZSBS catalog code: BS CL13
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: dog
TISSUE/ORGAN: thymus
Susceptibility to viruses: calicivirus * feline rhinotracheitis * herpes simplex * parvovirus * pseudorabies * reovirus * retroviruses
PROPERTIES: virology; C-type viruses growth
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1430, ECACC: 90110521, ICLC: AL97003
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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