380's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

380 (human, peripheral blood, leukemia, pre-B cell)

ICLC catalog code: HTL99019
Continuous culture, grown as suspension
round, small cells morphology
SPECIES: human (male, 15 years old) Karyology: (8;14) and (14;18) translocations
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, pre-B cell
DEPOSITOR: Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: EBNA negative

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 1-2 x 10^6 cells/ml; split 1:2-1:3; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
the cells are very difficult to culture, grow very slowly and should be frozen at not less than 5 x 10^6 cells/ampoule
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: DSMZ: ACC 39

Bibliographic references:

Proc Natl Acad Sci USA 1984;81:7166-7170 - PMID: 6334305

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105