Caki-2's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Caki-2 (human, kidney, carcinoma)

SPECIES: human
TISSUE/ORGAN: kidney
TUMOR: carcinoma

DSMZ catalog code: ACC 54
Morphology: epithelial-like cells, growing as adherent monolayer
Description: human kidney carcinoma established from the primary renal carcinoma of a 69-year-old man confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% McCoy's 5A + 10% FBS change medium twice every week, split confluent cultures 1:2 to 1:3 weekly using trypsin/EDTA, upon thawing grow cells immediately in 80 cm 2 ; seed out at ca. 2 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 cell harvest of ca. 1 x 106 cells/25 cm 2; doubling time of ca. 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)

Availability in cell line catalogues: ATCC: HTB 47, IZSBS: BS TCL8, ICLC: HTL95029

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;49:5
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

Caki-2 (human, Caucasian, kidney, carcinoma)

ICLC catalog code: HTL95029
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 69 years old)
TISSUE/ORGAN: kidney
TUMOR: carcinoma
tumorigenic in nude mice
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
McCoy's 5A + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2 Passage: 52 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, MD, NP, PepB)

Availability in cell line catalogues: IZSBS: BS TCL8, DSMZ: ACC 54

Bibliographic references:

Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
J Natl Cancer Inst 1977;49:5
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105