Ca Ski's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Ca Ski (human, Caucasian, cervix, carcinoma, epidermoid)

SPECIES: human, Caucasian
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epidermoid

ECACC catalog code: 87020501
Morphology: Epithelial
Endogenous viruses: contains human papilloma type 16
Description: Human Caucasian cervical epidermoid carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Beta Human chorionic gonadotropin (beta-hCG) Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 251; Karyotype: Hypertriploid, modal no 78
Derived from an epidermoid carcinoma of the cervix metastatic to the small bowel mesentery of a 40 year old Caucasian. Ca Ski cells secrete the ß subunit of human chorionic gonadotropin (ß-h Ch), express tumour-associated antigen and GbPD type B. Ca Ski cells have been reported to contain an integrated human papilloma virus 16 genome (HPV-16 about 600 copies per cell) and HPV-18 related sequences.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1550

Bibliographic references:

Science 1977;196:1456-1458 - PMID: 867042

Ca Ski (human, Caucasian, cervix, carcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 40 years old)
TISSUE/ORGAN: cervix
TUMOR: carcinoma
non tumorigenic
Endogenous viruses: contains human papilloma type 16
PROPERTIES: beta-HCG secr.; tumorigenicity

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5%CO2, split 1-3

Availability in cell line catalogues: ATCC: CRL 1550, ECACC: 87020501

Bibliographic references:

Science 1977;196:1456-1458 - PMID: 867042

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105