SPECIES: rat
TUMOR: leukemia, basophilic
DSMZ catalog code: ACC 147
Morphology: 1/4 adherent (epithelial-like cells), 3/4 in suspension (small round cells)
Description: rat basophilic leukemia this rat basophilic leukemia (RBL) cell line was established from chlorethylamine-treated rats; the cells were described to show marked variations in differentiation dependent on the growth rate and the cell cycle phases confirmed as rat with IEF of AST, NP
Viruses: ELISA. reverse transcriptase negative
Depositor: obtained from ATCC (CRL 1378), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS (cells may also be grown, but less efficiently, with MEM) maximal split ratio of 1:4; cells grow partly adherent, but trypsin is not needed to detach the cells at 37C with 5% CO2 about 46 x 106 cells/175 cm 2;
doubling time of ca. 25 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (74(69-76); rodent marker chromosomes present) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of AST, NP)
Availability in cell line catalogues: ATCC: CRL 1378, ECACC: 86061001, ICLC: ATL01001
Bibliographic references:
- J Exp Med 1974;139:600-616 - PMID: 4812630
- Nature New Biol 1973;244:73-75 - PMID: 4516143
SPECIES: rat
TUMOR: leukemia, basophilic
ECACC catalog code: 86061001
Morphology: Lymphoblast
Description: Rat basophilic leukaemia granulocyte
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10-20% Fetal Bovine Serum (FBS). Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37C.
Exhibits marked variations in differentiation when monitored for basophilic granule development, histamine content and membrane receptor sites for IgE.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1378, DSMZ: ACC 147, ICLC: ATL01001
Bibliographic references:
- J Exp Med 1974;139:600-616 - PMID: 4812630
- Nature New Biol 1973;244:73-75 - PMID: 4516143
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: rat
TUMOR: leukemia, basophilic
PROPERTIES: differentiation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1378, ECACC: 86061001, DSMZ: ACC 147, ICLC: ATL01001
Bibliographic references:
- J Exp Med 1974;139:600-616 - PMID: 4812630
- Nature New Biol 1973;244:73-75 - PMID: 4516143
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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