GDM-1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

GDM-1 (human, peripheral blood, leukemia, acute myelomonocytic)

SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myelomonocytic

DSMZ catalog code: ACC 87
Morphology: single round to oval cells in suspension
Description: human acute myelomonocytic leukemia established in 1979 from the peripheral blood of a 66-year-old woman with a Philadelphia chromosome negative myeloproliferative disorder (resembling chronic myeloid leukemia, CML, in blast crisis) after transformation to refractory acute myelomonocytic leukemia (AML) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujiasaki Cell Center, Okayama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS cells are very difficult to culture! maintain at 0.5-0.8 x 106 cells/ml; cells grow very slowly, split carefully at 37C with 5% CO2 maximum density at 1.2 x 106 cells/ml; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hyperdiploid; 48(46-48)<2n>XX, +8, +13, -16, +m, t(2;11)(q36;q13), del(6)(q21), t(7;?)(q35;?), del(12)(p13)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ICLC: HTL01008

Bibliographic references:

Leuk Res 1982;6:743-752 - PMID: 6396552

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105