SPECIES: rat
TISSUE/ORGAN: adrenal
TUMOR: pheochromocytoma
DSMZ catalog code: ACC 159
Morphology: small cells growing in clumps in suspension, adhering poorly to plastic
Description: rat adrenal pheochromocytoma established from a transplantable rat adrenal pheochromocytoma in 1976; cells were described to synthesize catecholamines (dopamine, norepinephrine); in response to nerve growth factor (NGF) a neuronal phenotype could be induced reversibly confirmed as rat with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from ATCC (CRL 1721), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% RPMI 1640 + 10% horse serum + 5% FBS split saturated culture 1:2 to 1:3 every 3-4 days; cells grow in suspension without collagen; using collagen (Collagen S- Roche at 30 ęg/ml) cells grow adherent, faster and to a higher density; the adherent cells may be detached mechanically without trypsin, for single cells the suspension should be passed several times through a syringe with a 22-gauge needle; seed out at ca. 5-15 x 106 cells/25 cm 2 at 37C with 5% CO2 about 1-2 x 106 cells/ml;
doubling time of ca. 50-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as rat with IEF of MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CRL 1721, ECACC: 88022401, ICLC: ATL98004, IZSBS: BS TCL 91
Bibliographic references:
- EMBO J 1983;2:643 - PMID: 6641712
- Methods Enzymol 1984;1147:207
- Proc Natl Acad Sci U S A 1976;73:2424-2428 - PMID: 1065897
- Science 1985;229:393-395 - PMID: 3839317
SPECIES: rat, New England Deaconess Hospital strain
TISSUE/ORGAN: adrenal medulla
TUMOR: pheochromocytoma
ECACC catalog code: 88022401
Morphology: With collagen - neuronal fibroblasts; without collagen - spherical clusters
Description: Rat adrenal phaeochromocytoma
Depositor: Obtained from ATCC, USA
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Fetal Bovine Serum (FBS). Adheres poorly to plastic; grows in small clusters. Use collagen coated flasks for attachment. Feed 3 times a week. Grows satisfactorily in suspension in untreated flasks. Maintain between 2-5x100,000 cells/ml; 5% CO2; 37C.
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1721, DSMZ: ACC 159, ICLC: ATL98004, IZSBS: BS TCL 91
Bibliographic references:
- EMBO J 1983;2:643 - PMID: 6641712
- Methods Enzymol 1984;1147:207
- Proc Natl Acad Sci U S A 1976;73:2424-2428 - PMID: 1065897
- Science 1985;229:393-395 - PMID: 3839317
ICLC catalog code: ATL98004
Continuous culture, grown as suspension
clusters of small cells morphology
SPECIES: rat
TISSUE/ORGAN: adrenal
TUMOR: pheochromocytoma
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: the cells are described to synthesize catecholamines (dopamine, norepinephrine); respond reversibly to NGF by induction of the neuronal phenotype.
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI + 5% FBS + 10% HS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split 1:3-1:6; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
to obtain single cells, pass the suspension through a 22G syringe; use collagen-coated flasks for attachment
Availability in cell line catalogues: ECACC: 88022401, DSMZ: ACC 159, IZSBS: BS TCL 91
Bibliographic references:
- Proc Natl Acad Sci USA 1976;73:2424-2426
- Science 1985;229:393-395 - PMID: 3839317
Monoclonal continuous culture, grown as monolayer
chromaffin cells morphology
SPECIES: rat, New England Deaconess Hospital strain
TISSUE/ORGAN: adrenal medulla
TUMOR: pheochromocytoma
tumorigenic in syngeneic animals
PROPERTIES: catecholamine prod.; neurotransmitter prod.; NPP prod.; dopamine prod.; differentiation; neurobiochemistry; neurobiology
Available in the following LABORATORY:
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Universita' degli Studi (PGUPG, Perugia)
RPMI 1640 + 10% HS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2 Passage: 20
Differentiation induced by NGF.
Availability in cell line catalogues: ATCC: CRL 1721, ECACC: 88022401, DSMZ: ACC 159, ICLC: ATL98004, IZSBS: BS TCL 91
Bibliographic references:
- EMBO J 1983;2:643 - PMID: 6641712
- Methods Enzymol 1984;1147:207
- Proc Natl Acad Sci U S A 1976;73:2424-2428 - PMID: 1065897
- Science 1985;229:393-395 - PMID: 3839317
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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