SPECIES: mouse, albino
TUMOR: neuroblastoma
DSMZ catalog code: ACC 148
Morphology: about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
Description: mouse neuroblastoma established from the spontaneous tumor of a strain A albino mouse confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: obtained from ATCC (CCL 131), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS + 1x non-essential amino acids split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm 2 at 37C with 5% CO2 about 8 x 106 cells/75 cm 2 ; maximal density at ca. 20 x 106 cells/175 cm 2;
doubling time of ca. 70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 131, ICLC: ATL99007, IZSBS: BS TCL 128
Bibliographic references:
- J Cell Biol 1969;43:69a
- Proc Natl Acad Sci U S A 1970;65:129-136 - PMID: 5263744
ICLC catalog code: ATL99007
Monoclonal continuous culture, grown as monolayer
neuronal-like and round, mostly loosely attached cells, some in suspension morphology
SPECIES: mouse, albino
TUMOR: neuroblastoma
DEPOSITOR: Dr G. Angelini, Biotechnology Dept, IST at ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce large quantities of microtubular protein, which plays a role in a contractile system which is responsible for axoplasmic flow in nerve cells
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split subconfluent cultures 1:3-1:6; seed at 2-4 x 104 cells/cm2; 5% CO2, 37°C
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Availability in cell line catalogues: DSMZ: ACC 148, IZSBS: BS TCL 128
Bibliographic references:
- J. Cell Biol. 1969, 43:69A; Proc: Natl Acad. Sci. USA 1970, 65:129-136
Continuous culture, grown as monolayer
neuron-like and amoeboid-like morphology
SPECIES: mouse, albino
TUMOR: neuroblastoma
tumorigenic in syngeneic animals
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
PROPERTIES: microtubular protein prod.; tumorigenicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 131, DSMZ: ACC 148, ICLC: ATL99007, IZSBS: BS TCL 128
Bibliographic references:
- J Cell Biol 1969;43:69a
- Proc Natl Acad Sci U S A 1970;65:129-136 - PMID: 5263744
Continuous culture, grown as monolayer
neuron-like and amoeboid-like morphology
SPECIES: mouse, albino
TUMOR: neuroblastoma
tumorigenic in syngeneic animals
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
PROPERTIES: microtubular protein prod.; tumorigenicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 131, DSMZ: ACC 148, ICLC: ATL99007, IZSBS: BS TCL 128
Bibliographic references:
- J Cell Biol 1969;43:69a
- Proc Natl Acad Sci U S A 1970;65:129-136 - PMID: 5263744
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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