LLC-PK1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

LLC-PK1 (pig, kidney)

SPECIES: pig
TISSUE/ORGAN: kidney

ECACC catalog code: 86121112
Description: Porcine kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims.
Properties: Products: Plasminogen activator Applications: Production of plasminogen activator

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Medium 199 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 196; Cells have been used in the production of plasminogen activator.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1392, IZSBS: BS CL 111

Bibliographic references:

In Vitro 1976;12:670-677 - PMID: 828141

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105