Caco-2's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Caco-2 (human, Caucasian, colon, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma

DSMZ catalog code: ACC 169
Morphology: epithelial adherent cells; after splitting cells start to grow in colonies
Description: human colon adenocarcinoma established from the primary colon tumor (adenocarcinoma) of a 72-year-old Caucasian man in 1974 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. A. Bacher, Technical University of Munich, Munich, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% MEM (with Earle's salts) + 20% FBS + 1x non-essential amino acids cells grow slowly; split confluent culture 1:6 to 1:10 every 5-7 days using trypsin/EDTA or, if necessary, a cell scraper; after thawing cells might start slowly to take up growth; seed out at ca. 1 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 about 3-6 x 106 cells/80 cm 2 or ca. 1.0 x 105 cells/cm 2; doubling time of ca. 80 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiologcal culture, RNA hybridization, PCR assay;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

Caco-2 (human, Caucasian, colon, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma

ECACC catalog code: 86010202
Morphology: Epithelial
Description: Human Caucasian colon adenocarcinoma
Viruses: See Properties
Depositor: Dr J Clarke, AVRI, Pirbright, UK
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). The addition of Bovine Insulin has been reported to be useful where there has been slow growth of cells. Split sub-confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. NB: During routine subculture the cells should always be subcultured before they achieve confluence.
Passages: 20; Karyotype: Hypertetraploid, modal No 96
Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: HTB 37, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

Caco-2 (human, Caucasian, colon, adenocarcinoma)

ICLC catalog code: HTL97023
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: modal number 96
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
DEPOSITOR: Dr A. Corsini, Pharmacological Sciences Institute, Milan, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: lipid production; small bowel biology; metabolism; lipoprotein

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. After thawing, cells might grow slowly. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR - mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 16,18; FGA: 19; TH01: 6; D18S51: 12; D21S11: 30; D8S1179: 12) * isoenzymes (confirmed as human with MD, NP)

Availability in cell line catalogues: ECACC: 86010202, DSMZ: ACC 169, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

Caco-2 (human, Caucasian, colon, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: modal number 96
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: lipid prod.; small bowel biology; metabolism; lipoprotein; tumorigenicity; cell biology; biochemistry; biophysics

Available in the following LABORATORY:

Pharmacia Upjhon (MIECC, Nerviano (MI))
MEM (EBSS) + 20% FBS + 1% non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 2-3

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

Caco-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

Istituto di Scienze Farmacologiche, Universita' degli Studi (MIUFC, Milano)
MEM + 15% FBS + Antibiotics + 1% non-essential amino acids + 20mM Tricine + 1mM Na pyruvate; Freezing medium: Culture medium + 20% FBS + 10% Glycerol; 37C, 5% CO2
Split 1:4 weekly.
Validated by: biochemical assays * cellular assays

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

Caco-2 (human, Caucasian, colon, adenocarcinoma)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: modal number 96
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: lipid prod.; small bowel biology; metabolism; lipoprotein; tumorigenicity; cell biology; biochemistry; biophysics

Available in the following LABORATORY:

Istituto Superiore di Sanità (RMSTC, Roma)
DMEM + 5% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: DMEM + 20% FBS + 10% DMSO; 37C, 5% CO2, split 1-9 , mycoplasma negative

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

Caco-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105