SPECIES: human
TISSUE/ORGAN: lung, fetal
ECACC catalog code: 84101801
Morphology: Fibroblast-like
Susceptibility to viruses: adenovirus * echovirus * herpes simplex * measles * poliovirus 1 * poliovirus 2 * poliovirus 3 * rubella * vesic. stomatitis (Indiana)
Description: Human fetal lung
Viruses: See Properties
Depositor: Dr P Jacobs, NIBSC, London, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: rhinoviruses, production of viral vaccines
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 14;
Karyotype: 2n = 46, diploid
Established from normal lung tissue of a 14 week old male fetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 171, NIA: AGO5965B, ICLC: HL95001
Bibliographic references:
- Nature 1970;227:168-170 - PMID: 4316953
- Nature 1972;238:26 - PMID: 4342691
- Nature 1972;239:316
ICLC catalog code: HL95001
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: human (male, 14 weeks old) Karyology: diploid
TISSUE/ORGAN: lung, fetal
Susceptibility to viruses: herpes simplex * poliovirus 1 * vesic. stomatitis (Indiana)
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: vaccine development; senescence; virology
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:4-1:6 using trypsin/EDTA; the cells are difficult to disaggregate after tripsinization; seed at 2-4x104 cells/cm2. 37C, 5% CO2. Passage: 21 , mycoplasma negative, HOECHST and PCR
Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 15; FGA: 21,23; TH01: 8; D18S51: 15,21; D21S11: 31.2; D8S1179: 13) * isoenzymes (confirmed as human with MD, NP)
Availability in cell line catalogues: ECACC: 84101801, ECACC: 97112601
Bibliographic references:
- Nature 1970;227:168-170 - PMID: 4316953
- Nature 1972;238:26 - PMID: 4342691
- Nature 1972;239:316
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: human (male, 14 weeks old) Karyology: diploid
TISSUE/ORGAN: lung, embryonic
Susceptibility to viruses: adenovirus * echovirus * herpes simplex * measles * poliovirus 1 * poliovirus 2 * poliovirus 3 * rubella * vesic. stomatitis (Indiana)
PROPERTIES: vaccine development; senescence; virology
Available in the following LABORATORY:
-
Universita' degli Studi (PRUMB, Parma)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 35
Availability in cell line catalogues: ATCC: CCL 171, NIA: AGO5965B, ECACC: 84101801, ICLC: HL95001, ECACC: 97112601
Bibliographic references:
- Nature 1970;227:168-170 - PMID: 4316953
- Nature 1972;238:26 - PMID: 4342691
- Nature 1972;239:316
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: human (male, 14 weeks old) Karyology: diploid
TISSUE/ORGAN: lung, embryonic
Susceptibility to viruses: adenovirus * echovirus * herpes simplex * measles * poliovirus 1 * poliovirus 2 * poliovirus 3 * rubella * vesic. stomatitis (Indiana)
PROPERTIES: vaccine development; senescence; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 171, NIA: AGO5965B, ECACC: 84101801, ICLC: HL95001, ECACC: 97112601
Bibliographic references:
- Nature 1970;227:168-170 - PMID: 4316953
- Nature 1972;238:26 - PMID: 4342691
- Nature 1972;239:316
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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