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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

6-23 (clone 6) (rat, WAG/RIJ, thyroid, medullary, carcinoma)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 26; Derived from non-clonal line rMTC 6-23 originally established from a transplantable WAG/RIJ rat medullary thyroid carcinoma. The cell line synthesises and secretes both neurotensin and calcitonin. Its clonal derivation demonstrates that a population derived from a single type of C-cell has the capability of producing both peptides. Secretion of these peptides has been shown to be stimulated by calcium, KC1, glucagon and catecholamines.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CRL 1607, IZSBS: TCL 130

Bibliographic references:

• Endocrinology 1980;107:509 - PMID: 6248326
  
• Proc Natl Acad Sci U S A 1980;77:3741 - PMID: 6932046