B9's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

B9 (mouse / mouse, hybridoma)

SPECIES: mouse / mouse
TUMOR: hybridoma

DSMZ catalog code: ACC 211
Morphology: round cells growing in suspension
Description: mouse hybridoma cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer; cells are IL6-dependent confirmed as mouse with IEF of AST, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. L. Aarden, Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS + 50 覡 mercaptoethanol + 50 pg/ml human IL-6 split saturated culture 1:3 to 1:4 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml, best in 24- or 6-well plate at 37C with 5% CO2 maximal density at about 0.8 x 106 cells/ml; doubling time of ca. 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (apparently murine chromosome morphology with extensive centric fusions) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ICLC: HYL98004, ECACC: 96080128

Bibliographic references:

Eur J Immunol 1987;17:1411-1416 - PMID: 3500054

B9 (mouse, BALB/c, hybrid, B cells x myeloma, hybridoma)

ICLC catalog code: HYL98004
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, BALB/c
TISSUE/ORGAN: hybrid, B cells x myeloma
TUMOR: hybridoma
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
PROPERTIES: fusion partner: SP2/0-Ag14; non producer hybridoma; IL-6 dependent

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 50uM 2-mercaptoethanol + 50pg/ml human IL-6 ; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 3-9 x 10^5 cells/ml; split 1:3-1:4; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
the cells grow very slowly, particularly after thawing
Validated by: isoenzymes (confirmed as mouse with AST, NP)

Availability in cell line catalogues: DSMZ: ACC 211, ECACC: 96080128

Bibliographic references:

Eur J Immunol 1987;17:1411-1416 - PMID: 3500054

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105