A-704's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

A-704 (human, kidney, adenocarcinoma)

ICLC catalog code: HTL95007
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 78 years old) Karyology: diploid to hyperdiploid
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
non tumorigenic
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: chemotherapeutic agents; cytotoxicity; tumorigenicity

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x105 cells/cm2. 37C, 5% CO2. Passage: 53 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, MPI)

Availability in cell line catalogues: IZSBS: TCL 117

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
Oncogene 1994;9(3):939-942 - PMID: 8108138
Proc Natl Acad Sci USA 1987;84(22):7972-7976 - PMID: 2825172

A-704 (human, kidney, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 78 years old) Karyology: diploid to hyperdiploid
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
non tumorigenic
Cell line available under some conditions.
PROPERTIES: chemotherapeutic agents; cytotoxicity; tumorigenicity

Available in the following LABORATORY:

Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
MEM (EBSS) + 15% FBS + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 3-4. Passage: 65 , mycoplasma negative
Validated by: isoenzymes (Me-2, 1; PGM3, 1; PGM1, 1; ES D, 1; AK1, 1; GLO-1, 2; G6PD,B)

Availability in cell line catalogues: ATCC: HTB 45, ICLC: HTL95007, IZSBS: TCL 117

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
Oncogene 1994;9(3):939-942 - PMID: 8108138
Proc Natl Acad Sci U S A 1987;84(22):7972-7976 - PMID: 2825172

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105