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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
  Karyotype: 2n = 46, diploid
  Established in 1963 from Burkitt's lymphoma in an 11 year old Negro male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. The cells should be handled under laboratory containment level 2.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, DSMZ: ACC 319

Bibliographic references:

• J Clin Pathol 1965;18:261
  
• J Natl Cancer Inst 1966;37:547 - PMID: 4288580
  
• Lancet 1964;1:238
  
• Trans N Y Acad Sci 1966;29:61
  

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
  RPMI 1640 + 20% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 120 , mycoplasma negative, culture
  Obtained from American Tyoe Collection,Rockville, Md (USA).
  

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, ECACC: 85011429, DSMZ: ACC 319

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Russian Academy of Medical Sciences (MWIIW, Moscow)
  mycoplasma negative
  

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

• J Clin Pathol 1965;18:261
• J Natl Cancer Inst 1966;37:547 - PMID: 4288580
• Lancet 1964;1:238
• Trans N Y Acad Sci 1966;29:61

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Centro Trapianto Midollo Osseo e Divisione Ematologia (PSHTM, Pesaro)
  RPMI 1640 + 10% FBS + 2mM L-Glutamine + Antibiotics + 1mM Na pyruvate; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2
  

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

• J Clin Pathol 1965;18:261
• J Natl Cancer Inst 1966;37:547 - PMID: 4288580
• Lancet 1964;1:238
• Trans N Y Acad Sci 1966;29:61

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Istituto Superiore di Sanità (RMSVI, Roma)
  RPMI 1640 + 15% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-6
  

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

• J Clin Pathol 1965;18:261
• J Natl Cancer Inst 1966;37:547 - PMID: 4288580
• Lancet 1964;1:238
• Trans N Y Acad Sci 1966;29:61

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Russian Academy of Sciences (SPBIC, St. Petersburg)
  
  Positive for Epstein-Barr nuclear antigen, but does not contain the EB virus
  

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

• J Clin Pathol 1965;18:261
• J Natl Cancer Inst 1966;37:547 - PMID: 4288580
• Lancet 1964;1:238
• Trans N Y Acad Sci 1966;29:61