TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, pre-B cell
DSMZ catalog code: ACC 20
Morphology: round to elongated, single cells in suspension
Description: human B cell precursor leukemia established from the peripheral blood of a 45-year-old man with chronic myeloid leukemia (CML) in blast crisis in 1980; contains the t(9;22) b2-a2 fusion gene confirmed as human with IEF of G6PD, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS cells are difficult to culture! cells appear to grow better in 24-well-plates than in flasks; maintain at 0.5-1.0 x 106 cells/ml; cells grow slowly, split 1:2 to 1:3 every 3-4 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at 2-3 x 106 cells/ml;
doubling time of ca. 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid; 47(46-48)<2n>X/XY, +mar, der(22)t(9;22)(q34;q11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13+ CD19+ CD37- HLA-DR+ cyIgG/M-) * isoenzymes (confirmed as human with IEF of G6PD, MDH, NP) * reverse transcriptase (negative)
Availability in cell line catalogues: ICLC: HTL99018
- J Natl Cancer Inst 1983;70:447-453 - PMID: 6572735
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.