ACHN's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

ACHN (human, Caucasian, kidney, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma

ECACC catalog code: 88100508
Morphology: Fibroblast
Description: Human renal adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Interferon and interferon-induction studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 4; Derived from the pleural effusion of a 22 year old male patient with metastatic renal adenocarcinoma. The cells were reported to be growth inhibited by human interferons.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1611, IZSBS: BS TCL68, ICLC: HTL99007

Bibliographic references:

Experientia 1987;43(4):435-437 - PMID: 3569495
FEBS Lett 1990;274(1-2):136-140 - PMID: 1701397
FEBS Lett 1991;295(1-3):17-21 - PMID: 1722462
Int J Cancer 1991;48(4):598-604 - PMID: 2045203
J Immunol 1991;147(1):149-154 - PMID: 1904900
J Interferon Res 1983;3(4):379-385 - PMID: 6672111
Japanese Journal of Cancer and Chemotherapy 1985;12(10):1983-1989
Japanese Journal of Cancer and Chemotherapy 1987;14(Pt1):699-705
Jpn J Cancer Res 1987;78(2):193-201 - PMID: 3030986
Proc Natl Acad Sci U S A 1987;84(22):7972-7976 - PMID: 2825172

ACHN (human, Caucasian, kidney, adenocarcinoma)

IZSBS catalog code: BS TCL68
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: human, Caucasian (male, 22 years old)
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: interferon assay; interferon induction

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 9 , mycoplasma negative, culture

Availability in cell line catalogues: ATCC: CRL 1611, ECACC: 88100508, ICLC: HTL99007

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105