MDA-MB-231's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

MDA-MB-231 (human, Caucasian, breast, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma

ECACC catalog code: 92020424
Morphology: Epithelial
Description: Human Caucasian breast adenocarcinoma
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L-15 + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 37C. CO2 is not required.
Passages: 35; Karyotype: Modal No.'s 62 and 64, near triploid
Isolated from pleural effusions of a breast cancer patient.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: HTB 26, ICLC: HTL99004

Bibliographic references:

J Natl Cancer Inst 1974;53:661-674 - PMID: 4412247

MDA-MB-231 (human, Caucasian, breast, adenocarcinoma)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 51 years old) Karyology: mean chromosomal number 68
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: specialized product secr.; estrogen receptor negative; chemotherapeutic agents; hormone sensibility; cell biology; chemosensibility

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro (GEIAP, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3 Passage: 32
Derived from pleural effusion

Availability in cell line catalogues: ATCC: HTB 26, ECACC: 92020424, ICLC: HTL99004

Bibliographic references:

J Natl Cancer Inst 1974;53:661-674 - PMID: 4412247

MDA-MB-231 (human, Caucasian, breast, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 51 years old) Karyology: mean chromosomal number 68
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: specialized product secr.; estrogen receptor negative; chemotherapeutic agents; hormone sensibility; cell biology; chemosensibility

Available in the following LABORATORY:

Dip. Scienze Biomediche ed Oncologia Umana (TOUCI, Torino)
L-15 + 10% FBS; Freezing medium: Culture medium + 5% or 10% DMSO; 37C, 5% CO2, split 1-2 Passage: 28

Availability in cell line catalogues: ATCC: HTB 26, ECACC: 92020424, ICLC: HTL99004

Bibliographic references:

J Natl Cancer Inst 1974;53:661-674 - PMID: 4412247

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105