G-361's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

G-361 (human, Caucasian, skin, malignant melanoma)

Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 31 years old) Karyology: subtetraploid
TISSUE/ORGAN: skin
TUMOR: malignant melanoma
tumorigenic in nude mice
PROPERTIES: melanin prod.; cell biology; tumorigenicity; hormone studies

Available in the following LABORATORY:

Dip. Scienze Biomediche e Biotecnologie, Universita' degli Studi (BGEBS, Brescia)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: MEM + 10% FBS + 8% DMSO; 37C, 5% CO2 , mycoplasma negative, HOECHST

Availability in cell line catalogues: ATCC: CRL 1424, ECACC: 88030401, ICLC: HTL99001

Bibliographic references:

Pediatr Res 1978;12:485

G-361 (human, Caucasian, skin, malignant melanoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: skin
TUMOR: malignant melanoma

ECACC catalog code: 88030401
Morphology: Epithelial
Description: Human Caucasian malignant melanoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Melanin Applications: Hormone studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Cells are slow in attaching.
Passages: 36; Karyotype: Triploid, Modal No 69
Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1424, ICLC: HTL99001

Bibliographic references:

Pediatr Res 1978;12:485

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105