SPECIES: mouse, DBA/2
TISSUE/ORGAN: blood
TUMOR: leukemia, lymphocytic
ECACC catalog code: 87092804
Morphology: Lymphoblast
Description: Mouse DBA/2 lymphocytic leukaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Cytotoxicity studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Fischer's + 2mM Glutamine + 10% Horse Serum (HS) or ATCC CRCM30 + 10% Horse Serum (HS). Maintain cultures between 5x10,000 to 8x100,000 cells/ml; 5% CO2; 37C.
Passages: 380;
Karyotype: 2n = 40, diploid
Established from a tumour induced in an 8 month old female 234 sub-line 212 of a DBA mouse. Originally sub-cultured in DBA/2 mice it was adapted to suspension culture. The line has been extensively used in cytotoxicity assays. Develops massive tumours in nude mice after a latent period of 7-10 days.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 219, ICLC: ATL99002
Bibliographic references:
- J Natl Cancer Inst 1949;10:179-192
- J Natl Cancer Inst 1966;36:405-421
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, DBA/2 (female, 8 months old)
TISSUE/ORGAN: ascitic fluid
TUMOR: leukemia, lymphocytic
tumorigenic in syngeneic animals and nude mice
PROPERTIES: chemotherapeutic agents; cytotoxicity
Available in the following LABORATORY:
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Istituto Ricerche Farmacologiche Mario Negri (MIILC, Milano)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + Hepes buffer + 2-mercaptoethanol; Freezing medium: Culture medium + 20% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
Doubling time 12 h. Seeding concentration 1 x 105 cells/ml; split when 6 x 105 cells/ml. Every 2-3 weeks centrifuge and resuspend in fresh medium. Tumor induced in vivo by methylcholanthrene.
Availability in cell line catalogues: ATCC: CCL 219, ECACC: 87092804, ICLC: ATL99002
Bibliographic references:
- J Natl Cancer Inst 1949;10:179-192
- J Natl Cancer Inst 1966;36:405-421
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, DBA/2 (female, 8 months old)
TISSUE/ORGAN: ascitic fluid
TUMOR: leukemia, lymphocytic
tumorigenic in syngeneic animals and nude mice
PROPERTIES: chemotherapeutic agents; cytotoxicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 219, ECACC: 87092804, ICLC: ATL99002
Bibliographic references:
- J Natl Cancer Inst 1949;10:179-192
- J Natl Cancer Inst 1966;36:405-421
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, DBA/2 (female, 8 months old)
TISSUE/ORGAN: ascitic fluid
TUMOR: leukemia, lymphocytic
tumorigenic in syngeneic animals and nude mice
PROPERTIES: chemotherapeutic agents; cytotoxicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 219, ECACC: 87092804, ICLC: ATL99002
Bibliographic references:
- J Natl Cancer Inst 1949;10:179-192
- J Natl Cancer Inst 1966;36:405-421
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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